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retroviral particles  (ATCC)


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    Structured Review

    ATCC retroviral particles
    Retroviral Particles, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/retroviral+particles/293+c18/pm41413567-155-5-10
    Average 95 stars, based on 200 article reviews
    retroviral particles - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Retroviral:

    Article Title: Stem cell specific mechanisms ensure genomic fidelity within HSCs and upon aging of HSCs
    Article Snippet: .. Cell-free supernatants containing retroviral particles were generated by transient transfection of Phoenix-gp packaging cells (ATCC number: CRL-3215) using Calcium Phosphate Transfection kit (Invitrogen). ..

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression.
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID:Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID:CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [11], GagPolS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Article Title: The transplantation of neural stem cells and predictive factors in hematopoietic recovery in irradiated mice.
    Article Snippet: A number of surprising observations have shown that stem cells, in suitable conditions, have the ability to produce a whole spectrum of cell types, regardless, whether these tissues are derived from the same germ layer or not.. This phenomenon is called stem cell plasticity, which means that tissue-specific stem cells are mutually interchangeable.. In our experiments, as a model, we used neural stem cells (NSCs) harvested from fetal (E14–15) neocortex and b-galactosidase positive.

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID: Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID: CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [ ], GagPolDS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Article Title: Diagnosis of Sexually Transmitted Diseases
    Article Snippet: .. Cells: (a) HEK293T (ATCC CRL-11268) and Phoenix GP cells are adherent cells used for the production of lentiviral and retroviral particles, respectively. (b) SupT1 (ATCC CRL-1942), a T-cell line readily infected by HIV-1 ( see Note 6 ). ..

    Article Title: Local environmental factors determine hematopoietic differentiation of neural stem cells.
    Article Snippet: Stem cells exhibit unique properties and hold high therapeutic promise, but factors influencing their differentiation after transplantation need to be recognized and defined for this promise to be fully met.. Here, we demonstrate that endogenous colony-forming unit spleen (CFU-S) colonies are not generated in lethally irradiated mice transplanted with neural stem cells obtained from brain tissue of syngeneic donors.. We investigated the proportion of transplanted neural stem cells that contributed to hematopoietic reconstitution and compared the distribution of transplanted cells in nonsplenectomized to that of splenectomized mice following sublethal whole-body irradiation.

    Generated:

    Article Title: Stem cell specific mechanisms ensure genomic fidelity within HSCs and upon aging of HSCs
    Article Snippet: .. Cell-free supernatants containing retroviral particles were generated by transient transfection of Phoenix-gp packaging cells (ATCC number: CRL-3215) using Calcium Phosphate Transfection kit (Invitrogen). ..

    Transfection:

    Article Title: Stem cell specific mechanisms ensure genomic fidelity within HSCs and upon aging of HSCs
    Article Snippet: .. Cell-free supernatants containing retroviral particles were generated by transient transfection of Phoenix-gp packaging cells (ATCC number: CRL-3215) using Calcium Phosphate Transfection kit (Invitrogen). ..

    Plasmid Preparation:

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression.
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID:Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID:CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [11], GagPolS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID: Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID: CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [ ], GagPolDS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Infection:

    Article Title: Diagnosis of Sexually Transmitted Diseases
    Article Snippet: .. Cells: (a) HEK293T (ATCC CRL-11268) and Phoenix GP cells are adherent cells used for the production of lentiviral and retroviral particles, respectively. (b) SupT1 (ATCC CRL-1942), a T-cell line readily infected by HIV-1 ( see Note 6 ). ..

    Labeling:

    Article Title: Local environmental factors determine hematopoietic differentiation of neural stem cells.
    Article Snippet: Stem cells exhibit unique properties and hold high therapeutic promise, but factors influencing their differentiation after transplantation need to be recognized and defined for this promise to be fully met.. Here, we demonstrate that endogenous colony-forming unit spleen (CFU-S) colonies are not generated in lethally irradiated mice transplanted with neural stem cells obtained from brain tissue of syngeneic donors.. We investigated the proportion of transplanted neural stem cells that contributed to hematopoietic reconstitution and compared the distribution of transplanted cells in nonsplenectomized to that of splenectomized mice following sublethal whole-body irradiation.



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